Bovine Rhinotracheitis Virus a Wɔde Nyarewa Ba a Ɛko Tia Nkwammoaa ELISA Detection Kit

Nhwɛsode a Ɛto so:VD105E
Abbr.:IBRV Ab Elisa na ɔkyerɛwee
Nhwɛsode: serum

Nkyerɛmu

Nsɛm a Wɔakyerɛkyerɛ mu

BVDV Ab Elisa na ɔkyerɛwee

Sɛnea Wɔabɔ Wɔn Tirim sɛ Wɔde Di Dwuma: Ɛyɛ ɔyare mmoawaBovine Rhinotracheitis Virus a Ɛyare noWɔde Antibody ELISA Detection Kit di dwuma dekyerɛ rhinotracheitis a ɛde ɔyare ba nommoawa a wɔko tia nyarewa a ɛwɔ nantwi mogya mu wɔ vitro.

Nhwɛsode: serum a wɔde hyɛ nipadua mu

Nneɛma a Wɔde Ma:

Infectious Bovine Rhinotracheitis Virus Antibody ELISA Detection Kit

Sɔhwɛ Nhyehyɛe & Nkyerɛase

ANAMMƆN 1 Sɔhwɛ Nhyehyɛe

1.1 Nsiesiei a wɔde hohoro nneɛma ho

Working adwene a wɔde si biribi so washing nsuo:Ansa na wɔde bedi dwuma no, ɛsɛ sɛ wɔsan de aduru a wɔde hohoro nneɛma no ba dan mu hyew (20-25℃) na wɔwosow no pɛpɛɛpɛ ma ɛyɛ pɛ. Afei fa nsuo a wɔatew ho fra ahohoro aduru a wɔahyɛ no den no mmɔho 20 (sɛ nhwɛsoɔ no, wɔde 5ml a wɔde hohoro nsuo a wɔahyɛ no den gu nsuo a wɔatew ho 95ml mu), fa fra yie, na wobɛtumi de nsuo a wɔde hohoro a wɔahyɛ no den no asie wɔ digrii 2-8 nnafua 7.

1.2 Nhwɛsoɔ Ahosiesie

1 Wɔfaa mmoa mogya mũ nyinaa, de centrifuged guu mu wɔ 4000rpm simma 10, na wɔboaboaa nsu a ɛwɔ soro ano. Wobetumi nso ayɛ serum coagulated wɔ abɔde mu a hemolysis nni mu.

2 Fa serum a wobɛsɔ ahwɛ no hyɛ mu mpɛn 50 wɔ serum dilution plate no mu (sɛ nhwɛso no: fa serum 3ul a wobɛsɔ ahwɛ no gu 147ul sample diluent mu).

Hyɛ no nsow: Ɛho nhia sɛ wɔde nnuru a wɔde siw ano a enye ne nea ɛyɛ papa no hyɛ mu

1.3 Sɔhwɛ kwan a wɔfa so yɛ

1Fa enzyme label Microplate (sɛnea nhwɛsode dodow te, wobetumi apaapae na wɔde adi dwuma mpɛn pii), fa serum a wɔafrafra no 100ul gu enzyme label Microplate no mu, hyehyɛ abura abien biara ma negative control ne positive control, na fa 100ul ka reaction well no ho. Wosow nhwɛsode no brɛoo wɔ abura no mu, fa sealing plate film no kata prɛte no so, na fa to digrii 37 so simma 30.

2Bue sealing plate film no, wosow solution a ɛwɔ plate well no mu no, fa 300μl a ɛyɛ adwuma concentration washing liquid gu abura biara mu, na afei tow nsu no gu abura no mu. Tia atifi hɔ anammɔn no mu mpɛn 5, na awiei koraa no, fa krataa a ɛho tew a ɛtwetwe nsu so bɔ wo ho ban.

3Diluted enzyme conjugate:Dilute enzyme conjugate (80×) mpɛn 80 (sɛ nhwɛso no: 10ul enzyme conjugate concentrate + 790ul sample diluent, dodow pɔtee a ɛho hia no, wobu akontaa sɛnea sɔhwɛ nhwɛso dodow te), fra yiye na fa to nkyɛn.

4Fa Enzyme Conjugate 100μl gu abura biara mu, kata prɛte no so, na fa to 37℃simma 30.

5Washing enzyme plate, oprehyɛn pɔtee no yɛ pɛ sɛ atifi hɔ 2.

6Fa 100μl substrate solution gu abura biara mu, kata prɛte no so na fa simma 10 to 37℃a ɛne hann ntam kwan ware.

ANAMMƆN 2 Nea efi mu ba no Nkyerɛase

Wɔkenkan abura biara OD450 bo wɔ abura 96 microplate akenkanfo no so.

2.1 Validation OD450nm boɔ a ɛwɔ negative control mu no yɛ < 0.25 na OD450nm boɔ a ɛwɔ positive control no mu no yɛ Kɛseɛ sene anaa ɛne 0.6 yɛ pɛ, nhwehwɛmu no mu aba no yɛ nokware. Anyɛ saa a, ɛsɛ sɛ wɔsan yɛ sɔhwɛ no bio.

2.2 Ɔkwan a wɔfa so bu akontaa

Wɔ nhwɛsoɔ biara mu no, fa nhwɛsoɔ ne control values a wɔateɛteɛ no bu S/P no sɛdeɛ ɛdidi soɔ yi:

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2.3 Nea efi mu ba no Nkyerɛase

Sɛ S/P < 0.2 a, wobu no sɛ ɛyɛ bɔne.

Sɛ S/P > 0.3 a, wobu no sɛ ɛyɛ papa.

Sɛ 0.2 sua anaasɛ ɛyɛ pɛ S/P Ennu anaasɛ ɛyɛ pɛ 0.3 a, wobu no sɛ ɛyɛ adwenem naayɛ, na wɔhyɛ nyansa sɛ wɔnsan fa nhwɛsode na wɔnsɔ nhwɛ dapɛn biako akyi.

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